Cleaning of Sendai Virus-infected Mice by Embryo Transfer Technique
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چکیده
منابع مشابه
Optimizing the embryo transfer technique.
The technique of embryo transfer is very crucial and great attention and time should be given to this step. In order to optimize the embryo transfer technique, several precautions should be taken. The first and most important is to avoid the initiation of uterine contractility. This can be achieved by the use of soft catheters, gentle manipulation and by avoiding touching the fundus. Secondly, ...
متن کاملEnumeration of antigen-presenting cells in mice infected with Sendai virus.
Substantial progress has been made in understanding Ag presentation to T cells; however, relatively little is known about the location and frequency of cells presenting viral Ags during a viral infection. Here, we took advantage of a highly sensitive system using lacZ-inducible T cell hybridomas to enumerate APCs during the course of respiratory Sendai virus infection in mice. Using lacZ-induci...
متن کاملMonitoring in vivo changes in lung microstructure with ³He MRI in Sendai virus-infected mice.
Recently, a Sendai virus (SeV) model of chronic obstructive lung disease has demonstrated an innate immune response in mouse airways that exhibits similarities to the chronic airway inflammation in human chronic obstructive pulmonary disease (COPD) and asthma, but the effect on distal lung parenchyma has not been investigated. The aim of our study is to image the time course and regional distri...
متن کاملRibonucleic acid polymerase induced in cells infected with Sendai virus.
A ribonucleic acid (RNA)-dependent RNA polymerase was induced in chick embryo fibroblast cells after infection with Sendai virus (parainfluenza 1 virus). The enzyme was associated with the microsomal fraction of infected cells and reached maximum detectable activity at 18 hr after virus infection. The activity of the enzyme in vitro was dependent on the presence of added magnesium ions and all ...
متن کاملAdenylate-rich sequences in Sendai virus transcripts from infected cells.
Virus-specific complementary ribonucleic acid (RNA) from cells infected with Sendai virus was isolated by a procedure involving hybridization with virion RNA and isopycnic centrifugation of the RNA hybrids. The complementary RNA contained adenylate-rich sequences which sedimented at about 4S.
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ژورنال
عنوان ژورنال: Experimental Animals
سال: 1990
ISSN: 0007-5124,1881-7122
DOI: 10.1538/expanim1978.39.4_601